A novel hydrogen peroxide biosensor based on adsorption of horseradish peroxidase onto a nanobiomaterial composite modified glassy carbon electrode.

Abstract

In, this work, a novel hydrogen peroxide biosensor derived from maize-tassel (MT) and multiwalled carbon nanotube (MWCNT) composite was used to adsorb horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. Tile morphology and structure of the products were characterized by SEM, FTIR and UV-visible spectroscopy. The electrochemical and electrocatalytic performance of the HRP/MT- MWCNT/GCE was studied using voltammetrie and amperometric methods. The amperometric response of the biosensor varied linearly with concentration of H20; from 9 pM to 1 inM with detection limit of 4.0 pM (S/N = 3).Furthermore, the biosensor exhibited good reproducibility and stability.